Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAAFLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPATYLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDRNLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEF-AVKSERFEVVSSLARKVLDKAEDVKELV--DLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSLINDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCGGVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK 3FJ1 Chain:D ((4-333)) -----------QHITRMRREIDEIPEAVQRLLDHGAQDVARVAAVLR--LR--DPSFVATVARGSSDHVCTYLSYAAELLLG---LPVASLGPSVASVYDARLR--LDRALCLAVSQSGKSPDIVAMTRNAGRD--GALCVALTNDAASPLAGVSA-----HTIDIHA-GPELSVAATKTFVTSAVAGLMLLADWAEDDGLRAALGNLPETLAAASRI-DW-PEMRVAIGARPSLFTLGRGTSLAVSNEAALKFKETCQ--LHAESYSSAEVLHGPVSIVEEGFPVLGFAAGDA-AEAPLAEIADQIAAKG--ATVFATTGRVTR-----ARVLEHVRSGHALTDPLSLIVSFYSMVEAFASERGIDPDA---------------------

General information: TITO was launched using: RESULT: Template: 3FJ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2080 -140495 -67.55 -429.65 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -67.55 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3FJ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FJ1-query.scw PDB file : Tito_Scwrl_3FJ1.pdb: