Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAVAGTGYVGLSIAVLLSQ---QHQVMAVDIIPEKVELINQRKSPIQDDYIEKYLAK-KDLNLVATLDAKEAYQDADFVVIAAPTNYDPK----KNFFDTSAVESVIETALKY-NPDAIMVIKSTVPVGYTESARKKF-----QTENILFSPEFLRESKALYDNLYPSRIILGTDKNNERLVSAAETFARLLQEGALKENIDTLYMGFTEAEAVKLFANTYLALRVSYFNELDTYSEMKGLDTQAIIDGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYE--DIP--EELIGAIVRSNSTRKDFIADQVLKMVDYYDYSAHNIFDERVEKDIIIGIYRLTMKSNSDNFRQSSIQGIMKRIKAKGVRVVIYEPTLESGSTFF-------------GSSVINDLEEFKRLSNAIVANRYDNSLEDVK---------E---KVYTRDIFERD 2O3J Chain:A ((10-452)) SKVVCVGAGYVGGPTCAMIAHKCPHITVTVVDMNTAKIAEWNSDKLPIYEPGLDEIVFAARGRNLFFSSDIPKAIAEADLIFISVNTPTKMYGRGKGMAPDLKYVESVSRTIAQYAGGPKIVVEKSTVPVKAAESIGCILREAQKLKFQVLSNPEFLAEGTAMKDLANPDRVLIGGESS-PEGLQAVAELVRIYENWV-P-RNRIITTNTWSSELSKLVANAFLAQRISSINSISAVCEATGAEISEVAHAVGYDTRIGSKFLQASVGFGGSCFQKDVLSLVYLCESLNLPQVADYWQGVININNWQRRRFADKIIAELFN------------TVTDKKIAIFGFAFKKNTGDTRESSAIHVIKHLMEEHAKLSVYDPKVQKSQMLNDLASVTSAQDVERLITVESDPYAAARGAHAIVVLTEWDEFVELNYSQIHNDMQHPAAIFDGRLILDQ-

General information: TITO was launched using: RESULT: Template: 2O3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2007 4635 2.31 11.70 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 2.31 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_2O3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O3J-query.scw PDB file : Tito_Scwrl_2O3J.pdb: