Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRPFLLLEVLEALQVAIKP-AHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFGEAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQHLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLK-TVGELIGLAFQVRDDVLDVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFETE-----SIVSVVESLRING 3M9U Chain:B ((34-299)) ------------------------AQLAAAMTYSVLAGGKRLRPLLTVATMQSLGVTFVPERHWRPVMALELLHTYSLIHDDLPAMDNDALRRGEPTNHVKFGAGMATLAGDGLLTLAFQWLTATDLPATMQAALVQALATAAGPSGMVAGQAKDIQSEHVNLPLSQLRVLHKEKTGALLHYAVQAGLILGQ-APEAQWPAYLQFADAFGLAFQIYDDILDVVS-------------DEAKNTYPGKLGL----IGANQALIDTIHSGQAALQGLPTSTQRDDLAAFFSYFDTER---

General information: TITO was launched using: RESULT: Template: 3M9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1134 -35286 -31.12 -143.44 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -31.12 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_3M9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M9U-query.scw PDB file : Tito_Scwrl_3M9U.pdb: