Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEA-IRQSAVFLLFVGDLNRAEKGARLHTDTFQ-PQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTS----WSQRLAEQFGQAEPSSTRKNLEQKKLL 5HEI Chain:B ((1-240)) MNEAIRTIQDHRSIRQYTDEAVSDEHLDTIIQSAQSAASSINGQQVTIISVQDKEKKKKLSELAGNQAWIDQAPLFLIFCADFNRAKIAAELNDAPLGVTDGLESILVGATDAGISLEAATVAAESLGLGTVPIGGIRRKPLEVIELLDLPEYVFPVSGLVVGHPSDHSAKKPRLPQAAVHHRESYNHDLKSLIQDYDAEMAEYMKKRTNGADDRNWSQTVSAIYKTIYYPEVRAMLEKQ---

General information: TITO was launched using: RESULT: Template: 5HEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 952 -28043 -29.46 -119.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -29.46 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_5HEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HEI-query.scw PDB file : Tito_Scwrl_5HEI.pdb: