Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAELIINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAKAVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYYALSKEIIGLMKD---QETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN 4Q25 Chain:B ((22-119)) ------------------------------------------------------------------------------------------------------------------VRSHLLAMGGLVEKQVNDAVNALIDADSGLAQQVREIDDQINQMERNIDEECVRILARRQPAASDLRLIISISKSVIDLERIGDEASKVARRAIQLCE-------

General information: TITO was launched using: RESULT: Template: 4Q25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 297 -36314 -122.27 -382.25 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -122.27 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4Q25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q25-query.scw PDB file : Tito_Scwrl_4Q25.pdb: