Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQG-PVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDE--GHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQL-SPELQPVQLILCGLPTATVVG------------------------------LR---------------------H------------------Y---K--T--PEIQGDILLIHGEQDDITLLSDAIEWAKP--QKHPITILSGANHFFTGYL-KQLRQIITRFIIMK 3FOB Chain:C ((5-281)) ---------AKITVGTENQAPIEIYYEDH--GTGKPVVLIHGWPLSGR-SWE----YQVPALVEAGYRVITYDRRGFGKSSQPWEGYEYDTFTSDLHQLLEQL----ELQNVTLVGFSMGGGEVARYISTYGTD--RIEKVVFAGAVPPYLYKSEDHPEGALDDATIETFKSGVINDRLAFLDEFTKGFFAAGDRTDLVSESFRLYNWDIAAGASPKGTLDCITAFSKTDFRKDLEKFNIPTLIIHGDSDATVPFEYSGKLTHEAIPNSKVALIKGGPHGLNATHAKEFNEALLLFLKD-

General information: TITO was launched using: RESULT: Template: 3FOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 891 -35223 -39.53 -181.56 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : -39.53 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_3FOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FOB-query.scw PDB file : Tito_Scwrl_3FOB.pdb: