Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLFTLKEVSHYLIENKNMAWSITMCLVAVLLTIFILNLILGLLVHFFDYSEPIWWHALSPYFIALLLFIMVWSVVTELYILRKGGHSLAKQLKAR--RLVKGESTPEEHAALKITEHLAQTFSLNVPTLYVLPDEVGVNALTAGFHSNDIVIILTWGALQNLDELELYGLLGHEFNQILSGEAVENTKLKILYSGLTTFSQWGSKLAKQGFKRYSPGYKHKFETVFVAVGGVIWLAGSLGVLITRFIKYLTLSGRTFRNDQKTMRLLKNSTNAQTLLRIYVHHSGSQIHSAYSESIAHMCFANSLSPQSWMNIHPSIRERIYELN---PTLLQDLQLENLKKLRNRPLFILFHVLEESETEIYTPWSSPQPLPLLRLSPISFALNDAIKPLSSDIRRNKKRPELIQRALQTATGSREV------MVAILMIRQYREFIPQDAPVSHAIVDALLNLDGRIHIQIFHDACKNIGHMPASIARQFLTKLALIIQEDGEIGLLDALLLERVKYELNLMPLHLPTAFEEVKPQIVRLIDALLHVQQINSPNQLEVRKRILRSLLNPDEMHIYDEISDEPLDLAEILNDIAGLLLRDRLSILAIVEMCLWSDRIITQDELDVLELLYWRFGFESDEIVEQMQKKNSVMII 4ZFV Chain:A ((10-445)) ---------------------NVLDFTVLGLNSGTSMDGIDCALCHFYQKTPD------AP---------------MEFELLEYGEVPLAQPIKQRVMRMILEDTTS-PSELSEVNVILGEHFADAVRQFAAER----------NVDLSTIDAIASHG-------QTIWLLSMPEEGQVKSALTMAEGAILASRTGITSITDFRISDQA---------------------------AGRQGAPLIAFFDALLL-------------------HHPTKLRACQNIGG----------IANVCFIPP-------DVDGRRTDEYYDFDTGPGNVFIDAVVRHFTNGEQ----------EYDKDGAMGKRGKVDQELVDDFLKMPYFQLDPPKTTGREVFRDTLAHDLIRRAEAKGLSPDDIVATTTRITAQAIVDHYRRYAPSQ------------EID---EIFMCGGGAYN-PNIVEFIQQSYPNTKIMMLDEAG-----------------------VPAGAKEAITFAWQGMEALVG-RSIPVPTRVETRQHYVLGKVSPG--LNYRSVMKKGMAFGGDAQQLP------------------WVSEMIVKKKGKVITN-NWAGSHHHH---------------

General information: TITO was launched using: RESULT: Template: 4ZFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2600 -72714 -27.97 -171.09 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -27.97 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_4ZFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZFV-query.scw PDB file : Tito_Scwrl_4ZFV.pdb: