Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MKKPVPKIYRTTNWSSYNQALIKRGNISIWFDPKTQWYAQPQG---KHGRNQTYSDTAIQCCL--------MIKSLFR------LSLRMVTGFA--------QSLIKLCG----------------------LNWTAPDYSTVCRRKKHIDIAIS----YQQSRDGLHLLVDSTGLKFLGEGEWKRKKHQPEY-RRQWRKLHIGIDAKTLQIRAVQLTTNNVSDSQVLGDLLDQIPL-DERIDSVYTDGAYDTKHCRQVILDRDAHAVIPPR---------------KNAKPWKDKKLRSLERN-----ELLKTVKRLGRSLWKKW------------SGYHRRSLVETKMHCIK---- 1YT3 Chain:A ((1-375)) MNYQMITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQL-FDGEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQILAAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITAKLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLADWRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVEKAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLLNWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ

General information: TITO was launched using: RESULT: Template: 1YT3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1229 16121 13.12 59.93 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 13.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_1YT3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YT3-query.scw PDB file : Tito_Scwrl_1YT3.pdb: