Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLSKKTIIALVVAAIALILAICVFVWKKPQSSSSSQENSASTQDHPVEKAEVLPYLNLTETKANYAVPFCEKKNCIDVDIQTIKTQ---D----AWLNSWIEKNQAKVIQAQINLKKDLSLQQAINAYVKKSDEWQDKYSKNRAYELHIRTRIASQRNQYVLLQLALDSKQEEITIKDRYYFFVADRKLHKNLTLFDVLKKD-QQPTVHQIVQTAYQDWLKKQTAD-VK-KQ-APKTL-YWG-QADWFFDGE-GVGLHYQANQITKDA-PQLDIYLTTEQTKKILQPQ-VYEQMF 5JEN Chain:A ((62-271)) ------------------------------------------------------------IVKAITFIEIKEEKDQSSIDVKTPALSGLSNKELENSINEKYLKESQ----------------QLYKEFIQSTSKN-----KKGHLSIYSDYETVTDTPDLLSIRRNIE--TTQASSYTQSRYITIDKKNDILLTLKSLFKDERYIKVISQNIKEQMKQQMKEDPNKIYWLTDEDAEPFKTILPDQTFYITEDHKLVISFDEYEVAPGYMGVTEFTIPTGVISNLLVGERYIR---

General information: TITO was launched using: RESULT: Template: 5JEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 31706 38.62 163.43 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 38.62 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_5JEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JEN-query.scw PDB file : Tito_Scwrl_5JEN.pdb: