Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMKSPILRLRRLRRTKAIRGLLCENNFSVEDLVQPIFIEESITQPIPISTMPGIYRLPEQDIEQEVKALYALGIRCVMPFGIS--HHKDSMGSDTWSDTGLLVRMIKAIKSSCPEMIVTPDICFCEYTTHGHCGVMIHD-QISNDLTVENLIKQSLAAAKAGADMLAPSGMMDGQVGAIREALDQAGYHNVLIMAHAIKFASALYGPFRVAVD-SNLVGDRHSYQLDYANTRQALREAALDESEGADLLIVKPGMFYLDILSQLRAQTKLPLAAYQVGGEYAAIKFAAIAKALDERKTVIESITAFKRAGADVIITYFAKDIAIWHNEF 1W56 Chain:B ((11-330)) ------PYTRLRRNRRDDFSRRLVRENVLTVDDLILPVFVLDGVNQRESIPSMPGVERLSIDQLLIEAEEWVALGIPALALFPVTPVEKKSLDAAEAYNPEGIAQRATRALRERFPELGIITDVCLCPFTTHGQDGILDDDGYVLNDVSIDVLVRQALSHAEAGAQVVAPSDMMDGRIGAIREALESAGHTNVRVMAYSAKYASAYYGPFRDAVGS-----NKATYQMDPANSDEALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEFRAPTFVYQVSGEY-AMHMGAIQNGWLAESVILESLTAFKRAGADGILTYFAKQAA------

General information: TITO was launched using: RESULT: Template: 1W56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1689 -76138 -45.08 -246.40 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -45.08 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1W56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W56-query.scw PDB file : Tito_Scwrl_1W56.pdb: