Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQPIRWYQNIPVISWLTLRGKCGHCQHPISIRYPAVELLTMLCSLVVVMVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDRFTLTLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKL-LAALGAWMGPLMLPLIVLLSSLLGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG 4XOI Chain:B ((3-269)) VNIKQKMQELNNEINMQQTVIYQASQALNCCVDEEHG-----KGSLEEAEAERLLLIATGKRTLLIDELNKLKNEMPSKGSVTLSEIRLP--------LKADFVCSTVQKPDAANYYYLIILKAGAENMVATPLASTSNSLNGDALTFTT------TFTLQDVSNDFEINIEVYSLVQKKSAVRTSN--------------------FALV--------GSYTLSLSSVGN--TKFVLDKVPFLSSLEG----HIYLKIKCQV------------------------------

General information: TITO was launched using: RESULT: Template: 4XOI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 858 -28726 -33.48 -141.51 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -33.48 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_4XOI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XOI-query.scw PDB file : Tito_Scwrl_4XOI.pdb: