Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MNSFMQQSLKMKKILMMSMCGALLLTGCNKTSQQDQTVSK-EQKSTAQKTTEQRDSSCLQ-----IMVAMHTINQNSKIEDLNQINEKLKTCVPSLKSEEQLKLIEASTAMYQRFLKQDYTDKTARAFEAFG--YAVLEQKQDLKKVIQSQ---KKLFAQLSPRDQYL------------------LQHEGQAYIELLYQGEGMFTYRRQPNYLIDVFSKALPADQKEFLSRMAKDNQDIFYNDGALAVSWKELTERALFWEKFIQKYPKSYFINDAKLLFNEY---RYFIFF------GLDNTPVSNEYAPNTWFDPDALQQIRFLSTQSQSSLAKPAQKFLKFIATPVDERNKQFKIDLTDENGQK----------KSTYQIVHEQLEQLLKFDSPWNTEIYRDCHIDAVCIDTN----------------- 3ZRH Chain:A ((2-449)) LEVDFKKLKQIKNRMKKTDWLFLNACVGVVEGDLAAIEAYKSSGGDIARQLTADEVRLLNRPSAFDVGYTLVHLAIRFQRQDMLAILLTEVSQQAAKCIPAMVCPELTEQIRREIAASLHQRKGDFACYFLTDLVTFTLPADIEDLPPTVQEKLFDEVLDRDVQKELEEESPIINWSLELATRLDSRLYALWNRTAGDCLLDSVLQA--TWGIYDKDSVLRKALHDSLHDCSHWFYTRWKDWESWYSQSFG-LHFSLREEQWQEDWAFILSLASQPGASLEQTHIFVLAHILRRPIIVYGVKYYKTLGYTRFQGVYLPLLWEQSFCWKSPIALGYTR---------GHFSALVAMENDDVTITFLPLVDSERKLLHVHFLSAQELGNEEQQEKLLREWLDCCVTEGGVLVAMQKSSRRRNHPLVTQMVEKWLDRYRQIRP

General information: TITO was launched using: RESULT: Template: 3ZRH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1550 75530 48.73 211.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 48.73 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_3ZRH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZRH-query.scw PDB file : Tito_Scwrl_3ZRH.pdb: