Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCNKASSDLTDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKKIALTDLYFPQLGIHIEVDEGH-HFLRNSKMEYSLNQIDEPLY--S------ISQTESDAMREEDIISITGHKIFRVNVFKNQEGQPQNLENIHQQID----KIIEEIKTAKNKLIEASTFKEWNIETEYNPQTYIDLGRISLADNVVLKTTKDVCNCFGYSYKNYQRGGALHPYKKDTLIWFPRLYENKDWINTISPDGLTITEKSTDETITLKKLEEWKNGPQKRIVFARVKDNLSSRAMYRFMGLYEFQKADLKDGAVWKRVKCEVQTYSPKETKC 1VSR Chain:A ((7-122)) -------------------------------RLASLLTGQGLAFRVQDAS----LP-GRPDFVVDEYRCVIFTHGCFWHHHHCYL-------FKVPATRTEFWLEKIGKNVERDRRDISR-LQELGWRVLIVWE-----------CALRGREKLTDEALTERLEEWICGEG-------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VSR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 319 -16534 -51.83 -160.52 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -51.83 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_1VSR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VSR-query.scw PDB file : Tito_Scwrl_1VSR.pdb: