Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTDPLVSCENLTSIVNLEHLMTTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADKLTQCE-NVDVIFGPAYKGIPFVAATAVALSQ---THNKSVPWGFNRKEAKDHGEGGVLVGAAVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDRQERGQGE----LSAIQEVQKELEIPVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI 2PRZ Chain:B ((8-223)) ----------------------------YQKNFLELAIECQALRFGSFKLKSGRESPYFFNLGLFNTGKLLSNLATAYAIAIIQSDLKFDVIFGPAYKGIPLAAIVCVKLAEIGGSKFQNIQYAFNRKEA-------IIVGSALENKRILIIDDVMTAGTAINEAFEIISNAKGQVVGSIIALDRQEVVSTDDKEGLSATQTVSKKYGIPVLSIVSLIHIITYLEGRITAEEKSKIEQYLQTYG-

General information: TITO was launched using: RESULT: Template: 2PRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 989 -46361 -46.88 -230.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -46.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.644

(partial model without unconserved sides chains): PDB file : Tito_2PRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PRZ-query.scw PDB file : Tito_Scwrl_2PRZ.pdb: