Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDDQNIFAKILRGELPAIKLYEDDQVLAFMDIMPQADGHALVIPKTPAVTLLDLPPEAAAYTIQIVQKIAK-AMETALNLDGIVLMQLSGAAAGQTVPHVHFHLIPTNVHQLGKHAAQLGDQDKIKALAEKIKAAL 3O0M Chain:B ((8-114)) -------VFCAIVSGDAPAIRIYEDENFLGILDIRPFTRGHTLVIPKTHTVDLTDTPPETVAGMAAVGQRIARAARESGLHADGNNIAINDGKAAFQTVFHIHLHVVP------------------------------

General information: TITO was launched using: RESULT: Template: 3O0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 419 -64479 -153.89 -644.79 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -153.89 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.698

(partial model without unconserved sides chains): PDB file : Tito_3O0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O0M-query.scw PDB file : Tito_Scwrl_3O0M.pdb: