Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKKQSRLRRAKSTRLHIR-ALGVTRLCVNRTPRHIYAQVISADGGKVLAQASTLDASLR--S--GTTGNIEAATKVGALIAERAKAAGVTKVAFDRSGFKYHGRIKALADAAREGGLEF 5MMI Chain:P ((48-166)) --TRREDRTARHVRIRKKVEGTPERPRLCVFRSNKHLYVQVIDDTKMHTLAAASTMQKAISENIDYSAGPTVEVAQKIGEMIAKSCLEKGITKVAFDRGGYPYHGRVKALADAAREHGLVF

General information: TITO was launched using: RESULT: Template: 5MMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 468 -5441 -11.63 -47.72 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain P : 0.81 3D Compatibility (PKB) : -11.63 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.703

(partial model without unconserved sides chains): PDB file : Tito_5MMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MMI-query.scw PDB file : Tito_Scwrl_5MMI.pdb: