Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MLYQYHC---ACCDKVVASTDKSCP--YCGSHHIRSPYGLWMFCVMACLAVVVVFKVVHLYIQNHQDTPVQSTLLDVLNEGDKSTKQ 5SVA Chain:T ((37-127)) LSKVQLYEDLCRYEDTLSKLVESVDRFKPNLDIAKDLIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNECHDELKA

General information: TITO was launched using: RESULT: Template: 5SVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 103 -3677 -35.70 -44.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain T : 0.64 3D Compatibility (PKB) : -35.70 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.128

(partial model without unconserved sides chains): PDB file : Tito_5SVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5SVA-query.scw PDB file : Tito_Scwrl_5SVA.pdb: