Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDIYVEFRGKYKVDGESRDSEHKGWLEVNSWSHNIRQPKSATSSSVGGHTAERVEHSDMVFVKDLDATSPKLWEACSAGYTFDEVQIDFYRANGDKRIKYLQIKLKHVLVSSVTPTVNE-E-GVPTEAFGLKYAAVEWTYNQQDINGTAKG-AVTKKWSLSNNTASYAA 3EAA Chain:A ((2-162)) AFDTYIKLD-K--VDGESTDDKHKKWIEVLGFAWGAGNECTMESGT-QGLNTGKAMMSVLRVTKWMDCASVKLASAAVQGQNFPTLELEICTQA-GDKFAFCIYKFTHVAVSSYQCSGATGGSDRPQETIDFAYKEVTWEYVPQDQNGKAGGKIGPEGWSLITNKK----

General information: TITO was launched using: RESULT: Template: 3EAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 688 -43462 -63.17 -275.07 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -63.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_3EAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAA-query.scw PDB file : Tito_Scwrl_3EAA.pdb: