Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTSIAPVINQYGVTVSTY-SEIVEHLKEKYREIYGQDVYLENDSQDGQWIG---VIARVVADCNAAVADVYSSMSPSTAGTDALS-RNVKINGIRRAVATKSTVSVVLVGAAGTIINNGIVSDKNNNRWLLPTQVVI---PPEGEIFVAAS--AEKPGAILALPNSITTISTPTRGWQSVNNPQASTLGAPVESNVKLRQRQALSTAIPSRSYTEGILGALFSLDGVSRCKVYENKKSFVDPLELPPNSLSVVVAGGDDQLIAETIRVKKAPGCDLYGNTTVIRPTVYGDPVEIQYWRPVQKSIGLRFELTTNSDYTVDIGEQIKSATADYINQLDIGDRIAINKLYVPAGLYGALDARSYEIESLQLTVDGVPVEGDYTLDFNAVAYCDSDDIEISVAGGG 1G9M Chain:G ((5-321)) ------EVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVGAGSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGT------------------GPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSVNFTDNAKTIIVQLNTSVEINCTGAGHCNIS---------------RAKWNNTLKQIASKLREQFG-----------------NNKTIIFKQSS-----------GGDPEIVTHSFNCG---GEFFYCNSTQLFNSTWFN---------GSDTITLPCRIKQ----IINMWQKVGKAMYAPPISGQIRCSSNITGLLLTR------DGGNSNNESEIFRPGGGDMRDNWRSELYKYKVVKIE----------

General information: TITO was launched using: RESULT: Template: 1G9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1432 60517 42.26 205.14 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain G : 0.64 3D Compatibility (PKB) : 42.26 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.135

(partial model without unconserved sides chains): PDB file : Tito_1G9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G9M-query.scw PDB file : Tito_Scwrl_1G9M.pdb: