Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQIRPFPPTDFIDQADDEEATRLTPAPDLMDWVVKNYLTVDGELYNPDHDHIAELIHENEGFLAFAWASQACTVKKQMVSGQCEKVMFNVGGWRKARQEQQMRDWFGYVPVYLITIDASYCEQATDRDFCALIEHELYHIGVERDEDGEPLYSE---MTGLPKHYLAGHDVEEFVGVVKRWG-ADENVKRLIEVAKQAPFVSDVNISKCCGTCLIN 1JFU Chain:A ((5-180)) ----TGDPACRAAVATAQKIAPLAHGEVAALTMASAPLKLPDLAFEDADGKPKKLSDFRGKTLLVNLWATW-C--------VPCRKEMPALDELQGKLSGP---------NFEVVAINIDTRDPEKPKTFLKEANLTRLGYFNDQKAKVFQDLKAIGRALGMPTSVLVDPQGCEIATIAGPAEWASEDALKLIRAATG-------------------

General information: TITO was launched using: RESULT: Template: 1JFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 908 -41679 -45.90 -242.32 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -45.90 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_1JFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JFU-query.scw PDB file : Tito_Scwrl_1JFU.pdb: