Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLQYSSRLTVMEYPPTYPSVIPNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTTNHLIYPVFVLPGQNQTQDIPSMPNIQRLSADLLLKKAERLLELGVSKLALFPVTPQEDKSLTAEAAWREDGLVQTTCRLLKKELPEMVLITDGALDPYTTHGQDGIIDETGYVLNDETVECLIKQALSHAEAGAEVIAPSDMMDGRIGAIRQALEANGHIYTNIMAYSAKYASSFYGPFRDAVGSASNLKGGNKYNYQMDFANRAEALHEIALDIQEGADMVIVKPGMPYLDVVREVKDTFGIPTFIYQVSGEYAMLAGAIQNGWLS-DSVILESLMCCRRAGADGIWTYFAETAAEKLKEMN 2C1H Chain:B ((25-328)) ------------------------------------------------------LVQENTLTVNDLVFPLFVMPGTNAVEEVSSMPGSFRFTIDRAVEECKELYDLGIQGIDLFGIPEQ--KTEDGSEAYNDNGILQQAIRAIKKAVPELCIMTDVALDPFTPFGHDGLVKD-GIILNDETVEVLQKMAVSHAEAGADFVSPSDMMDGRIGAIREALDETDHSDVGILSYAAKYASSFYGPFRDALHSAPQF--GDKSTYQMNPANTEEAMKEVELDIVEGADIVMVKPGLAYLDIVWRTKERFDVPVAIYHVSGEYAMVKAAAAKGWIDEDRVMMESLLCMKRAGADIIFTYYAKEAAKKLR---

General information: TITO was launched using: RESULT: Template: 2C1H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1721 -170844 -99.27 -563.84 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -99.27 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_2C1H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C1H-query.scw PDB file : Tito_Scwrl_2C1H.pdb: