Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVKTENHLSVQQESPIDKTLSMIERIALDPSSDVAKLEKMIELQERVMKTQAKQAYDNAMVLAQSEMPPIDKFKKGHNSNYAPLDHIMSIVFPVLKRNNLFVRWTSDPKENGSLCVTCICSHVGGHSETSSMDVKEDRGGSKSDIQGMGSAFTYAKRYTLSALLGLVLTDDTDGARINLKVTDAQATMLRNKLKFFKPEALEAFKAKIGCEVEELPRGDFDYWCSYIDN---QINKLMANQGEKNANP 1QST Chain:A ((1-160)) -----LDFDILTN----D-------------GTHRNMKLLIDLKNIFSRQL--P----------------KMPKEYIVKLVF---DRHHESMVILKNKQKVIGGICFRQYK--------------PQRFAEVAFLAVTANEQVRGYGTRLMNKFKDHMQKQ-NIEYLLTYADN----------------------------FAIGYFKK-QGFTK--EHRMPQEKWKGYIKDYDGGTLMECYIHPYVDY--

General information: TITO was launched using: RESULT: Template: 1QST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 708 -57501 -81.22 -366.25 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -81.22 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_1QST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QST-query.scw PDB file : Tito_Scwrl_1QST.pdb: