Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEKFSVEKVLSVHRWTPTLFSFTMTRPSHFKFTAGQFARIGL-KVGDELIVRAYSVVSSPFDETLEFFSIVVPDGAFTSNLQHLKVGDELYLEKIPYGYLTLARYQQPLP-HDLWLLATGTGLAPFLSMLQDFETWSNYQKINLVYSVRTAAELAY----VDRIQE---IAETFGEGHLGFKFIPIITRDPSAPLHERLPVLIENGELEKVAGLELSPASSHVMLCGNPQMVDDTKEALKRCGLTMNRRGE-GNIAVENYW 4K1X Chain:B ((2-251)) ------DAQTVTSVRHWTDTLFSFRVTRPQTLRFRSGEFVMIGLLDDNGKPIMRAYSIASPAWDEELEFYSIKVPDGPLTSRLQHIKVGEQIILRPKPVGTLVIDAL---LPGKRLWFLATGTGIAPFASLMREPEAYEKFDEVIMMHACRTVAELEYGRQLVEALQEDPLIGELV-EGKL--KYYPTTTREEFHHMGRITDNLASGKVFEDLGIAPMNPETDRAMVCGSLAFNVDVMKVLESYGLREGANSEPREFVVEKY-

General information: TITO was launched using: RESULT: Template: 4K1X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1300 -30425 -23.40 -126.77 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -23.40 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_4K1X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K1X-query.scw PDB file : Tito_Scwrl_4K1X.pdb: