Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLNLMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHMHFGPQHEAEGLPTHLKRTILLVFSITLHNI-PEGLAVGVAFGAAATADNPTAAILA-AVSVALGIGIQNFP----EGAAVSIPLRQEGLSRKKAFVYGQASG-----IVEPIAGVIGALLVTKVELLLPYALAFAAG-------AMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG--- 3O7O Chain:A ((1-264)) MVVLMTKPGTSDFVWNGIPLSMELNLWN---------IKEYSGSVAMKFDGE---KITFDADIQNLSPKEPERYVLGYPEFYYGY---------------KPWENHTAEGSKLPVPVSSMKSFSVEVSFDIHHEPSLPLNFAMETWLTREKYQTEASIGDVEIMVWFYFNNLTPGGEKIEEFTIPFVLNGESVEGTWELWLAEWGWDYLAFRLKCPVKKGRVKFDVRHFLDAAGKALSSSARVKDFEDLYFTVWEIGTEFGS--PETKSAQFGWKFENFSIDLEVREHHHHHH

General information: TITO was launched using: RESULT: Template: 3O7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1379 -122900 -89.12 -505.76 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -89.12 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_3O7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O7O-query.scw PDB file : Tito_Scwrl_3O7O.pdb: