Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKNEIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIA-PNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNT-TDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK--- 2AN1 Chain:A ((4-292)) HFKCIGIVGHTTHEMLYRWLCDQGYEVIVEQQIAHELQLKNVPTGTLAEIGQQ---ADLAVVVGGDGNMLGAARTLARYDINV-IGIN--RGNLGFLTDLDPDNALQQLSDVLE--------------------------------------------------------------GRYISEKRFLLEAQVCQQRISTAINEVVLHPGKVAHMIEFEVYID-------------------ETFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIRLRFS--HDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS-------YFNTLSTKLGWSKKLF

General information: TITO was launched using: RESULT: Template: 2AN1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1510 11077 7.34 41.18 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 7.34 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_2AN1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AN1-query.scw PDB file : Tito_Scwrl_2AN1.pdb: