Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKSEFTLGHVEKLIS----------DWISSRSEFTKIKQ---PIKFDMKKLLLNKSEIGNRDQYIRA--KGQEIIDSLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTEN--------------IILRTEYGNEIFAKYDEILKN--YLDWAWIIPVE------SGDAKKLERLLGDELINKKVPILNYYSHRQ------------ 3A25 Chain:A ((29-301)) IKPRIREILSKELPEELVKLLPKRWVRIGDVLLLPLRPELEPYKHRIAEVYAEVLGVKTVLRKGDYELLYGSDTVTVHVENGIKYKLDVAKIMFSPANVKERVRMAKVAKPDELVVDMFAGIGHL-SLPIAVYGKAKVIAIEKDPYTFKFLVENIHLNKVEDRMSAYNMDNRDFPGENIADRILMGYVVRTHEFIPKALSIAKDGAIIHYHNTVPEKLMPREPFETFKRITKEYGYDVEKLNELKIKRYAPGVWHVVLDLRVFKS

General information: TITO was launched using: RESULT: Template: 3A25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1104 2256 2.04 10.49 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 2.04 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_3A25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A25-query.scw PDB file : Tito_Scwrl_3A25.pdb: