Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLGANQKHKNYYEGPKVIVTWALGHLLGLKMPEDLNKEWQSW-------QMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIATDAGREGELVARWILEYVHF-NKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESALARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITLQAGK-------EQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEIQREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFAPEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRAR---YEKLSNEERKIYQMIVSRFLGLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETET---ETVKWEKGMRINP-DFAIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPKGKQLLVLVNP---SLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQEYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRKMEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAAALKAAMKK 1MW9 Chain:X ((20-580)) ----------YLGS-DYVVKSSVGHIRDLPT-----------ERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKA--DHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNI--DRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEA-GYITYMRTDSTNLSQDAVNMVRGYISDN--FGKK---YLP---ESPNQYA--------REAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPAL-EDRILPAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENNLDQVANHEAEWKAVLDHFFSDFTQQLDKAEK-------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1MW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2415 138331 57.28 276.11 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain X : 0.71 3D Compatibility (PKB) : 57.28 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_1MW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MW9-query.scw PDB file : Tito_Scwrl_1MW9.pdb: