Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGLIADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVLCLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAFTKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESMVRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEIDYINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE 3WFJ Chain:F ((4-309)) ---AIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQ-------------------LPIVL-------------IILFTKAMQLEKMLQDIQSL-----EVLCLLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNL----EAAAKKLADKL----LNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMVATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDYINGAISRKGKKYGVATPYCDFLTELVHAKEDSL---

General information: TITO was launched using: RESULT: Template: 3WFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1396 -220891 -158.23 -843.09 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain F : 0.86 3D Compatibility (PKB) : -158.23 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_3WFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFJ-query.scw PDB file : Tito_Scwrl_3WFJ.pdb: