Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEA---LNAILPMSFIPQGTDLLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNILHETIAPAAIDQDVIE-EAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD 3AW8 Chain:B ((12-351)) -----------------------RMLALAGYPLGLSFRFLDPSPEACAGQVGELVVGEFLDEG-ALLRFAEGLALVTYEFENVPVEAARRLEGRLPL-YPP--AKALEVAQDRLREKTFFQGLGVPTPPFHPVDGPEDLEEGLKRVGLPALLKTRRG------QALVRTEEEALEALKALGGRGLILEGFVPFDREVSLLAVRGRTGEVAFYPLVENRHWGGILRLSLAPAPGASEALQKKAEAYALRAMEALDYVGVLALEFFQVGEELLF-NEMAPRVHNSGHWTIEGAETSQFENHLRAVLGLPLGSTAPRGQSA-MVNLIGEK--PPFAEVLKVEGAHLHWYGKA-VRPGRKVGHIT-LRRDGLKALEE--------

General information: TITO was launched using: RESULT: Template: 3AW8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1785 -20108 -11.26 -60.93 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -11.26 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_3AW8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AW8-query.scw PDB file : Tito_Scwrl_3AW8.pdb: