Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKSKPIIIGVTGGSGSGKTSVSRAIFN----NFPDHSIMMLEQDSYYKDQSHLSFEERLNT-----NYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATIIQEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIEQYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK 1UEI Chain:B ((21-232)) ----EPFLIGVSGGTASGKSSVCAKIVQLLGQ-YRQKQVVILSQDSFYR---VLTSEQKAKALKGQFNFDHPDAFDNELILKTLKEITEGKTVQIPVYDFVSHSRKEETVTVYPADVVLFEGILAFYSQEVRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILN--

General information: TITO was launched using: RESULT: Template: 1UEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 963 26190 27.20 131.61 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 27.20 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_1UEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UEI-query.scw PDB file : Tito_Scwrl_1UEI.pdb: