Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEGQWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIE-AREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE 2ZDH Chain:D ((1-319)) MRVLLIAGGVSPEHEVSLLSAEGVLRHIPF---PTDLAVIAQDGRW----LLGEK--ALTALEAKAAPEGEHP-------FPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEPPVVPFDPPFFV----------KPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGEASPVGEVRYEAPFYDYETKYTPGRAELLIPAPLDPGTQETVQELALKAYKVLGVRGMARVDFFLA-EGELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELALT-------------

General information: TITO was launched using: RESULT: Template: 2ZDH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1872 -80360 -42.93 -252.70 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -42.93 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_2ZDH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZDH-query.scw PDB file : Tito_Scwrl_2ZDH.pdb: