Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 1UX1 Chain:B ((3-130)) ----RQELITEALKARDMAYAPYSKFQVGAALLTKDGKVYRGCNIENAAYSMCNHAEQTALFKAVSEGDTEFQMLAVAADTPGPVSPCGACRQVISELCTKDVIVVLTNLQGQIKEMTVEELLPGAFSSEDL

General information: TITO was launched using: RESULT: Template: 1UX1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 651 -77201 -118.59 -603.13 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -118.59 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_1UX1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UX1-query.scw PDB file : Tito_Scwrl_1UX1.pdb: