Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDAEAYLGPE-----DEAAHSYGLRRTPRVRAMY--EKPGTIYLYTMHTHRILNIITQPEGIPQGVMIRAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLV----FEKKK---TPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQI-EKDWGWRKENEKEGSINIFR--- 4HHT Chain:A ((1-229)) -----GIDELYKKEFGIVAGVDEAS--------RGCLAGPVVAAAVVLEKEIEGINDSRLLDEIMEKAAVGIGIASPEEIDLYNIFNATKLAMNRALENLSVKPSFVLVDGKGIELSVPGTCLVKGDQ--------KSKLIGAASIVAKVFRDRLMSEFHRMYPQFSFHKHKGYATKEHLNEIRKNGVLPIHRLSFEPVLELLTDDLLREFFEKGLISENRFERILNLLGARKSVVFRKER

General information: TITO was launched using: RESULT: Template: 4HHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1102 -14697 -13.34 -72.75 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -13.34 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.148

(partial model without unconserved sides chains): PDB file : Tito_4HHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HHT-query.scw PDB file : Tito_Scwrl_4HHT.pdb: