Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEATTLPQQFIKK-YRLLLGE-EASDFFSALEQGSVKKGFRWNPLKPAGLDMVQTYHSE--ELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSA-MIVGTAAAAKPGEKVL-DLCAAPGGKSTQLAAQM-KGKGLLVTNEIFPKRAKILSENIERWGVSNAIVTNHAPAELVPHFSGF-FDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILSSAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSVAGLEKTIRIWPHKDQGEGHFVAKLTFHG---QNQMHKEKKTRKKSKVQMTKEQEKLWTEF-SNDFHYEATGRLLVFNDHLWEVPELAPSLDG-LKVVRTGLHLGDFKKNRFEPSYALALATKKIENI-PCLPITQKEWQSYTAGETFQ--RDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFPKGLRFH--- 2FRX Chain:A ((7-474)) ------YFPDAFLTQMRE----AMPFDDFLAACQR-PLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFW--PLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISA-RMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVFGAAV-PEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFP----GANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAATGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALAS-PDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKL

General information: TITO was launched using: RESULT: Template: 2FRX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2458 148081 60.24 339.63 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 60.24 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_2FRX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FRX-query.scw PDB file : Tito_Scwrl_2FRX.pdb: