Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTSGFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDE-KRGQFARASWGIDYHDILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLITEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSYQTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPMLSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECL-TDVRPVIRGTAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK 5D6S Chain:D ((28-376)) MNIKLEIQKMAKEIGISKIGFTTADDFDYLEKSLRLGVEEGRTTGFEHKNIEERIYPKLSLESAKTIISIAVAYPHKLPQQPQKTEFKRGKITPNSWGLDYHYVLQDKLKRLAKGIEKLTENFEYK------GMVDTGALVDTAVAKRAGIGFIGKNGLVISKEYGSYMYLGELITNLEIEPDQEVDYGCGDCRRCLDACPTSCLIGDGTMNARRCLSFQTQDKGMMDMEFRKKIKTVIYGCDICQISCPYNRGIDNPL--DIDP--DLAMPELLPFLELTNKSFKETFGMIAGSWRGKNILQRNAIIALANLHDRNAIVKLMEIIDKNNNPIHTATAIWALGEIVKKPDEGMLDYM----------------------------

General information: TITO was launched using: RESULT: Template: 5D6S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1744 -5399 -3.10 -15.65 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -3.10 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_5D6S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D6S-query.scw PDB file : Tito_Scwrl_5D6S.pdb: