Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEKKAVMITKKAGEDMLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVFHPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEA-QQAMKDQLICDAKTIYAIQYWELLTKGK 3X0S Chain:A ((10-169)) -----------------------------ERTYLYRGRILNLALE---------GRYEIVEHKPAVAVIALRE-GRMLFVRQMRPAVGLAPLEIPAGLIEPGE--DPLEAAR-ELAEETGL-SGDLTYLFSYFVSPGFTDEKTHVFLAENLKEVEA--------AIEVVWMRPEEALERHQRGEVEFSATGLVGVLYYHAFLRG-

General information: TITO was launched using: RESULT: Template: 3X0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -66959 -96.21 -440.52 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -96.21 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_3X0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3X0S-query.scw PDB file : Tito_Scwrl_3X0S.pdb: