Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMYAIITYLLTRFTAIFHYVKTCQDMGKAKEKNSLAETPYNPSSPYSSTKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEGKNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQ--PK-----DAYDHVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVEAAYAKNGQ 1KEW Chain:B ((159-340)) -------------------------------------TAYAPSSPYSASKASSDHLVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHARALHMVVTEGKAGETYNIGGHNEKKNLDVVFTICDLLDEIVPKATSYREQITYVADRPGHDRRYAIDAGKISRELGWKP-LETFESGIRKTVEWYLANTQW-----------------

General information: TITO was launched using: RESULT: Template: 1KEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 718 6486 9.03 37.06 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 9.03 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_1KEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KEW-query.scw PDB file : Tito_Scwrl_1KEW.pdb: