Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMVKGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPEITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPDDIRSGKYFDENYKRVK 2GC9 Chain:A ((3-178)) TKTFKTLDDFLGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMVAGRWVTDQKADIVMLTEGIYKISWTEPTGTDVALDFMPNEKKLHGTIFFPKWVEEHPEITVTYQNEHIDLMEQSREKYATYPKLVVPEFANITYMGDAGQNNEDVISEAPYKEMPNDIRNGKYFDQNYHRLN

General information: TITO was launched using: RESULT: Template: 2GC9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -63260 -71.72 -359.43 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.95 3D Compatibility (PKB) : -71.72 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_2GC9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GC9-query.scw PDB file : Tito_Scwrl_2GC9.pdb: