Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRFAKDAELLQYMVPGSTLADIVSSEEDADDYDAYFDAPAAKAEGVSGVVPGEGSAGTGTNAQGVATSLSKFQPAQHKNADAKVLGRIHIDDYYDGHGSGGGASGGAQSNQRVLNELRDMTMSSRFAQNAASKNVDRSERATVENVLDPRTRLILYKLVNSGILSEINGCVSTGKEANVYYAVSGDGSPAALKVYKTSILSFKDRDQYVSGEFRFQRYCKSNPRKMVRTWAEKEARNLIRLQDGGVLAPAVKLL----RQHVLIMEFLGEDGWPAPRLKEV-TFPSAKALDKCYLDLCCTMRKMYARSHLIHGDLSEYNLLLYRGRVVVIDVSQSVEYDHPRSMSF------LRRDIVNVNSFFRSRGLTGLFTLQDLFHFITADPAKSGWQRCQGPPNDEELMARLKEIRDDYGEGGAVPVDAEQAKVDEQVFLNITVPRSLNEICDRAGPNKEVAVFVEGMTAAPAAAQTEDHSGADGEGDGDGDDEDSEADDADSPMKAKAAPQAVLANMTKEERKEHRKAVKEANREKRAEKKKNNANRKKKGRK 5EH0 Chain:A ((6-200)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------KGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQ-----------------------TLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGN--IDLNSWLKKKKSIDPWE-RKSYWKNMLEAVHTI-HQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDGTVNYMPPEAIKDMKISPKSDVWS-LGCI-------LYYMTYGKTPFQQ---------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 810 -35849 -44.26 -194.83 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -44.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_5EH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EH0-query.scw PDB file : Tito_Scwrl_5EH0.pdb: