Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHSLKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQKIFLGFSDTTMNHLML-HKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRI--SEIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFDNSRYADSTELCQKYKLFPDLSDW-EGKILLLETSEEKSKPEDFKKMLLTLKDTGIFAVINGLLVGKPMD--ETF-HDDYKEALLDII-DSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQEQVIRFDYNK 4IIY Chain:A ((42-365)) VGDTIGFFSSSAPATVT--AKNRFFRGVEFLQRKGFKLVSGKLTGKTDFYRSGTIKERAQEFNELVYNPDITCIMSTIGGDNSNSLLPFLD-YDAII--ANPKIIIGYADTTALLAGIYAKTGLITFYGPALIPSFGEHP-PLVDITYESFIKILTRKQSGIYTYTLPEKWSDESINWNENKILRPKKL-YKNNCAFYGS-GKVEGRVIGGNLNTLTGIW-GSEW----------M--PE---IRNGDILFIEDSRKS--IATVERLFSMLKLNRVFDKVSAIILGKHELFDCAGSKRRPYEVLTEVLDGKQIPVLDGFDCSHTHPMLTLPLGVKLAIDFDNKNISITEQ-

General information: TITO was launched using: RESULT: Template: 4IIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -57408 -31.46 -181.67 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -31.46 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4IIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IIY-query.scw PDB file : Tito_Scwrl_4IIY.pdb: