Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFEKIIQLKNCRYDYTLSPSV-KKFTLKDNTFFETKVGNYELTR-LLEKVPNSGEGFQLKIIINKELTGAKINIT-------------------------------DKFGLRLVDIFKSEDHHIHQEKFY---FLMDSLVERGVFTKSER-------------------- 1G13 Chain:A ((1-162)) ----SSFSWDNC--DEGKDPAVIRSLTLEPDPIIVP--GNVTLSVMGSTSVPLSSP-LKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

General information: TITO was launched using: RESULT: Template: 1G13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 233 8687 37.28 81.95 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 37.28 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_1G13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G13-query.scw PDB file : Tito_Scwrl_1G13.pdb: