Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKEKMFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGENIVEFEHYFKNQVNELLD-SNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLLVELISSYDSKINLEE----IVHNAQNSLFNVKQQLNVEEEEDLCL 3LFH Chain:B ((2-134)) ------KEKFVLIITHGDFGKGLLSGAEVIIGK--QENVHTVGLNLGDNIEVVRKEVEKIIKEK-LQEDKEIIIVVDLFGGSPFNIALSMMK-EYDVKVITGINMPMLVELLTSINVY-DTTELLENISKIGKDGIKVIEKSSL---------

General information: TITO was launched using: RESULT: Template: 3LFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 549 -50896 -92.71 -397.62 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -92.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_3LFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LFH-query.scw PDB file : Tito_Scwrl_3LFH.pdb: