Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQHLSKHRHISFTAHYTGYIWYQMGISHEALATSKGKSLAYLVHPLESWAEKYVGGSMRTTLKQRHTMLDHDLEKLIQENPDLQVLEIACGLSPRGWWFRQHYPSISYRELDLPDMAQAKQNALQQIEKNAPEVLSVDLFTEAFAQAFEVFDSNRPLVVISEGLINYFDKDLLKQLIQSIAHYGASFKKLHYLT-DLYPEPVKNKLASIIWNSSKLLKLMSRSSFSFHFKTPLEVKDFFKDAGFSQVNVEQPQIFFGQVSKDSDEEHLGDLVWTIHAQLK 4A6D Chain:A ((179-334)) ---------------------------------------------------------------------------------VFPLMCDLGGGAGALAKECMSLYPGCKITVFDIPEVVWTAKQHFSF--EEQIDFQEGDFFKDP----LPEA-----DLYILARVLHDWADGKCSHLLERIYHTCK--PGGGILVIESLLDEDRRGP--LLTQLYSLN-MLVQTE--GQERTPTHYHMLLSSAGFRDFQFKKT----------------------------

General information: TITO was launched using: RESULT: Template: 4A6D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 694 -68801 -99.14 -449.68 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -99.14 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_4A6D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A6D-query.scw PDB file : Tito_Scwrl_4A6D.pdb: