Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRLKCLGGPLYGQEY----SHAGDEF--------LFNDKQSGKQTLYRKQTLDFTPPQDVFVAESISNIIAYNLALQLKK 3EF2 Chain:B ((245-282)) --------GVMLGVNKAGDAAHAYNFTLTKDHADIVFFEPQNGGYLN----------------------------------

General information: TITO was launched using: RESULT: Template: 3EF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 34 -2867 -84.31 -110.25 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -84.31 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_3EF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EF2-query.scw PDB file : Tito_Scwrl_3EF2.pdb: