Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIIVALDAKSQYDALKIVEQLDPTLCRVKVGKELFTHEGPSVVKKLQEENFEVFLDLKFHDIPNTTAQAVCAAADLGVWMVNVHASGGRKMIETCVERLKAGN----YQTQLIAVTVLTSMGREDL-KDIGLDIEPVEQVKRLAKLTKESGLDGVVCSAQEAKILRELIGQDFSLVTPGIRPEGSNADDQKRIVTPKQAMLDGSTHLVIGRPITKAENPTEMLKSILASIA 2YYU Chain:B ((7-224)) --FIVALDFPSKQEVERFLRPFAGTPLFVKVGMELYYQEGPAIVAFLKEQGHAVFLDLKLHDIPNTVKQAMKGLARVGADLVNVHAAGGRRMMEAAIEGLDAGTPSGRMRPRCIAVTQLTSTDERMLHEELWISRPLVETVAHYAALAKESGLDGVVCSANEAAFIKERCGASFLAVTPGIRFADDAA----RVVTPRKARALGSDYIVIGRSLTRAADP------------

General information: TITO was launched using: RESULT: Template: 2YYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1074 -76488 -71.22 -365.97 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -71.22 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2YYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YYU-query.scw PDB file : Tito_Scwrl_2YYU.pdb: