Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLNSSIDKKMTTLNILFKTTSLCLAMALLVGCNDNDDNDSSTPAPRSNQTVNILAFNDFHGNLEPPKRYVEAANPNDASQTVRIPVGGVSYFADAIKKLKAQN----PNNVVVSAGDLISASPLTSSLFLDEPTIEVMNDIQIDFNAVGNHEFDRGTDELRRLQNGGCQQYTSTAPCQINKNFSGAKFNFLAANVAMKNDTSKTIFPAYKVKTYGGIPVAFIGLTLKATPSIVSAAGIKDVEFRDEADTVNALIPELQK-QGIEAIVVVVHEGAAPSTKLNQKTCDGLSGPILGILDRLN-PAVDIVVSGHTHQSYICDYA-------------TKNPAKPFLLTSAGQYGTLITDIKVGLDGKTGDIIKKDAKQIPVQSEAYTSGTTTVSLTDLYQKFSKTPSIEAILDKYRQAVTTISGRVVGTSTAVVSR--TQVESGESPLGDMIADAQQAAALQASNQGSDFTLMNPGGVRADLLINSSNQITFGDIFAVQPFGNSIVTLSLTGKQIRDVLEQQWSGANANSPRILQPSKELSYQYAANTSVSPRASNIMVAGSPLVDTKVYRVTVNSFLADGGDNFTVLKEGQNRV-GGGQDIDALESYVAQNSKDSPLIIPATTRIKVIK 1HP1 Chain:A ((4-500)) ----------------------------------------------DKTYKITVLHTNDHHGHFWRNEY----------------GEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPES-DLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTVLRQQEKW-------------------AKFPLLSANIYQKSTGE-RLFKPWALFKRQDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGEHGSN-----APGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCKPDQQNGIWIVQAHEWGKYVGRADFEFRNGE--MKMVNYQLIPVNLKKK-----RV---LYTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAAQMDRT------GADFAVMSGGGIRDSIE---AGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAVAQMKP-DSGAYPQFA-NVSFVAKD-----GKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPRLDNKPGYVNTGFIDAEVLKAYIQKSS-----------------

General information: TITO was launched using: RESULT: Template: 1HP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2865 105368 36.78 221.83 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 36.78 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_1HP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HP1-query.scw PDB file : Tito_Scwrl_1HP1.pdb: