Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRPGKKSSSNKSRVMKNKYAHRSWLGAVCLLGCSLSYAAEEQFNDALNAANSGNTALLDQYQLA-MQNDVLGYYPEYWKLNTNLGFQSPTSIVSFAQRYPQSAMAEKLAADYVEEKVKQADFASAQPILPYVS--NPDQAENCALAQVRAKSGD-ALVFAEYKDVWLATESQPESCIGL-------------GRMMLSS-PLMSTQDKQQRLWVQLRAGLSGQALATAQTLGL----NLSLA-------QLNQIQANP------LNYLWSAPKTNDVDYAYLIFALGRLANNDLGNAFANVQRVAQGTPESVQKYLYRTVAYIGGTTVMKNNFNREVLQYFDASYGYPL-SP-EEAEIYARQAIRFSAWESLIRAIDSMSVSQKQEDRWQYWLARATEQRGDSNSKNTAQCIYKKLAE-SGDDYHNLLAKDRLGVRY-NHQPYNDEPTASDLRRLDQNI--HFNRAFTLRRINVNPTYTNREWNWAVRQAYLQHDDG-LLLAAAKRAHDMGWYDRAIYAADRTTNKHNDTYRYVTPHKTNVVSHSYNAGIDPAWAYGLMRQESRFVTSARSHVGAGGLMQIMPDTAKLIARQMGETYNPAALSEMNTNIRYGTFYLSMIQGQLSNNPVLATAGYNAGPNRARRWQPDYQPIEADQYTETIPLLETRDYVKHVMTNATHYGVILGQGAQSLIQRMKVIPTRSSP 5G05 Chain:Y ((78-537)) ---------------------------------------QLLVYHADSLFHDKEYRNAVSKYTMALQQKKAL-----------------------------LPSEIEVKYKMAECYTMLKQDKDAIAILDGIPSRQRTPKINMMLANLYKKAGQERPSVTSYKEVLRQCPLALDAILGLLSLSVKGAEVASMTMNVIQTVPNLDWLSVWIKAYAFVHTGDNSRAISTICSLEKKSLLRDNVDLLGSLADLYFRAGDNKNSVLKFEQAQMLDPYLIK---GMDVYGYLLAREGRLEDVENLGCRLFNISDQHA-----EPWVVSGCH-SFY-SKRYSRALYLGAKAIQLNSNSVQALLLKGAALRNMGRVQEAIIHFREAIRLAPCRLDCYEGLIECYLASNSI---REAMVMANNVYKTLGANAQTLTLLATVCLEDPVTQEKAKTLLDKALTQRPDYIKAVVKKAELLSREQ-KYEDGIALLRNALA----NQSDCVLHRILGDFLVAVNEYQEAMDQYSIALS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5G05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 1774 -15111 -8.52 -38.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain Y : 0.56 3D Compatibility (PKB) : -8.52 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_5G05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G05-query.scw PDB file : Tito_Scwrl_5G05.pdb: