Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLIASLIAISSSSIFAADTTASAAKNDVFKQAEQLYAAKNYPAAFQEVQRLAQTG--NAQAIYNLGYMTQMGQGTAKDNAKALKYYEDASNKGYAQ--AS---YTLAQIYETGELGVAKDSNKFSQYIQKAAAQGS----DDATVKIATILFAQKKPQSHQIALQKLAPLIRKGNYPAI---QVKALYDISQGVENKNPLMKRQGIEALQTIAQKG--YAPASMALATMMANGNIIPQNLPQAKQIFTELAKQN-------------VPNARESLASVEKIIAEKNKQAAAAPTQPAPKK 2C0L Chain:A ((367-623)) -------------------------KHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTALMALAVSFTN----ESLQRQACEILRDWLRYTPAYAHLVPSKRILGS-LLS-----DSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSG---EYDKAVDCFTAALSVRPNDYLLWNKLGATLANG--------NQSEEAVAAYRRALELQPGYIRSRYNLGISCINLG----AHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAA---------

General information: TITO was launched using: RESULT: Template: 2C0L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 3977 4.01 18.50 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 4.01 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2C0L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C0L-query.scw PDB file : Tito_Scwrl_2C0L.pdb: