Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIAKLKREKRKDPLWGIILSIIVGSVL---MIYPLSYDISGWRPLFMLMIMLFWVVCQPTWCGVWFAFGMGIFTDLLLDAPLGLNALSFVIVTFITRFLIRERRILTFGNLWTIATLVIIAHLAFMWVTQTMGGIHFSIARHWQPLMTSILTWPVVYYCLAKWRI--------- 5IQJ Chain:A ((4-134)) -------MDERFNAALHESAHTVIAQVLGFNTATPIIYENSSTNPD------------EKHWLGKAFI-----------DTTNG-NVEDIALVGLAGEAIQYYIEGVD------------VGDCPFIWECNLED-ISLSDQELVKDLYNDVELWEKLYTLFEQHHDSILDLANSI

General information: TITO was launched using: RESULT: Template: 5IQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 482 -46765 -97.02 -392.98 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -97.02 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_5IQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IQJ-query.scw PDB file : Tito_Scwrl_5IQJ.pdb: