Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTYAYVRIEPNLQFEFSRYISFFSEYGYKIQKNRLVVEEVSVDKSIVYRDKFLNLISYSLEEGDILVIKSMDCLGNSFEEILDIVNKIDQKKIRLICLDYSKSEINGDLKIFFLHFLKLSAEFEKLFKSDKKNFKDHKPTRKVGRPEILNNEQKSRVIELFKKGYSVYSLAKEFSVTRTVIQRILNNASKTNIL 2GM5 Chain:C ((2-125)) -ALFGYARVS------LDIQVRALKDAGVKA--NRIFTDKAS-----SDRKGLDLLRM-KVKEGDVILVKKLDHLGRDTADMIQLIKEFDAQGVSIRFIDDG-ISTDSYIGKMVVTILSAVAQAERQRILER---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 482 -76332 -158.36 -658.03 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -158.36 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_2GM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM5-query.scw PDB file : Tito_Scwrl_2GM5.pdb: